Cell Culture

Cell culture is the process of growing and maintaining cells in vitro (outside the body in a laboratory setting) under controlled conditions, such as temperature, humidity, and nutrient supply. It is a valuable tool for studying the behavior of cells, developing new drugs and therapies, and producing biological products.

Cell cultures can be derived from various sources, including animal tissues, plants, and microorganisms. They can be maintained as two-dimensional monolayers or as three-dimensional structures, such as spheroids or organoids.

The process involves several steps, including cell isolation, CC medium preparation, CC vessel preparation, and cell seeding. After the cells are seeded, they are incubated under the appropriate conditions, and the medium is changed periodically to provide the necessary nutrients and remove waste products.

Cell culture is widely used in research, particularly in the fields of molecular biology, genetics, and biotechnology. It also has many practical applications, such as in the production of vaccines, recombinant proteins, and cell-based therapies.